RESISTANCE TO ACNU INDUCED TOXICITY IN TRANSGENIC TOBACCO SUSPENSION-CULTURES WITH ADA GENE TRANSFERRED FROM ESCHERICHIA-COLI

Citation
J. Briza et al., RESISTANCE TO ACNU INDUCED TOXICITY IN TRANSGENIC TOBACCO SUSPENSION-CULTURES WITH ADA GENE TRANSFERRED FROM ESCHERICHIA-COLI, Biologia plantarum, 35(1), 1993, pp. 125-129
Citations number
NO
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00063134
Volume
35
Issue
1
Year of publication
1993
Pages
125 - 129
Database
ISI
SICI code
0006-3134(1993)35:1<125:RTAITI>2.0.ZU;2-N
Abstract
The protein coding region of the E. coli DNA repair gene ada combined with the CaMV 35S promoter has been transferred to tobacco by means of Agrobacterium tumefaciens Ti plasmid. In transgenic plants having the ada gene in a sense orientation, detectable amounts of O6-alkylguanin e-DNA-alkyltransferase has been found whereas in non-transformed plant s this activity is absent. Cell suspension cultures derived from the f ormer plants showed lower sensitivity to the toxic (growth inhibiting) effects of the bifunctional alkylating agent 1-nitroso-3-(aminomethyl -1,3-diazinylo)-methylurea compared with cell cultures derived from a control non-transformed plant or from transgenic plants harbouring the ada gene in an opposite, non-sense orientation.