EFFECT OF ATP CONCENTRATION AND TEMPERATURE ON FIREFLY LUCIFERASE ACTIVITY

Authors
GALIS I JIRASKOVA J
Citation
I. Galis et J. Jiraskova, EFFECT OF ATP CONCENTRATION AND TEMPERATURE ON FIREFLY LUCIFERASE ACTIVITY, Biologia plantarum, 35(1), 1993, pp. 147-150
Citations number
NO
Categorie Soggetti
Plant Sciences
Journal title
Biologia plantarum → ACNP
ISSN journal
00063134
Volume
35
Issue
1
Year of publication
1993
Pages
147 - 150
Database
ISI
SICI code
0006-3134(1993)35:1<147:EOACAT>2.0.ZU;2-A
Abstract
The dependence of luciferase activity in the homogenate of leaves of t ransgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentrati on was between 0.625 mM. and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25-degrees-C and is completely lost during 30 min.