The dependence of luciferase activity in the homogenate of leaves of t
ransgenic tobacco plants with chimeric firefly luciferase gene on ATP
concentration and temperature was studied. The optimum ATP concentrati
on was between 0.625 mM. and 2.5 mM. The activity rapidly decreased if
the homogenate was kept in 25-degrees-C and is completely lost during
30 min.