PCR-BASED IMMUNOGENOTYPING AT THE IG HEAVY-CHAIN CDR3 LOCUS - IMPROVEMENTS IN RESOLUTION

Authors
POLLARD P OWEN G WORWOOD M
Citation
P. Pollard et al., PCR-BASED IMMUNOGENOTYPING AT THE IG HEAVY-CHAIN CDR3 LOCUS - IMPROVEMENTS IN RESOLUTION, British Journal of Haematology, 84(1), 1993, pp. 169-171
Citations number
8
Categorie Soggetti
Hematology
Journal title
British Journal of Haematology → ACNP
ISSN journal
00071048
Volume
84
Issue
1
Year of publication
1993
Pages
169 - 171
Database
ISI
SICI code
0007-1048(1993)84:1<169:PIATIH>2.0.ZU;2-6
Abstract
An improved electrophoretic separation of products after PCR employing consensus primers to amplify the CDR3 of the immunoglobulin heavy cha in variable region allows visualization of a ladder of fragments in po lyclonal samples of peripheral blood as well as a single band where th ere is clonal proliferation.