PURIFICATION AND CHARACTERIZATION OF AN OXYGEN-LABILE, NAD-DEPENDENT ALCOHOL-DEHYDROGENASE FROM DESULFOVIBRIO-GIGAS

Authors
HENSGENS CMH VONCK J VANBEEUMEN J VANBRUGGEN EFJ HANSEN TA
Citation
Cmh. Hensgens et al., PURIFICATION AND CHARACTERIZATION OF AN OXYGEN-LABILE, NAD-DEPENDENT ALCOHOL-DEHYDROGENASE FROM DESULFOVIBRIO-GIGAS, Journal of bacteriology, 175(10), 1993, pp. 2859-2863
Citations number
32
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
175
Issue
10
Year of publication
1993
Pages
2859 - 2863
Database
ISI
SICI code
0021-9193(1993)175:10<2859:PACOAO>2.0.ZU;2-W
Abstract
A NAD-dependent, oxygen-labile alcohol dehydrogenase was purified from Desulfovibrio gigas. It was decameric, with subunits of M(r) 43,000. The best substrates were ethanol (K(m), 0.15 mM) and 1-propanol (K(m), 0.28 mM). N-terminal amino acid sequence analysis showed that the enz yme belongs to the same family of alcohol dehydrogenases as Zymomonas mobilis ADH2 and Bacillus methanolicus MDH.