PRESENCE OF MRR-LIKE AND MCR-LIKE RESTRICTION SYSTEMS IN CORYNEFORM BACTERIA

Authors
VERTES AA INUI M KOBAYASHI M KURUSU Y YUKAWA H
Citation
Aa. Vertes et al., PRESENCE OF MRR-LIKE AND MCR-LIKE RESTRICTION SYSTEMS IN CORYNEFORM BACTERIA, Research in microbiology, 144(3), 1993, pp. 181-185
Citations number
9
Categorie Soggetti
Microbiology
Journal title
Research in microbiology → ACNP
ISSN journal
09232508
Volume
144
Issue
3
Year of publication
1993
Pages
181 - 185
Database
ISI
SICI code
0923-2508(1993)144:3<181:POMAMR>2.0.ZU;2-1
Abstract
Efficient transformation of Brevibacterium flavum MJ233C and Corynebac terium glutamicum ATCC 31831 (up to 5.0 x 10(7) transformants/mug DNA) depends on the source of plasmid DNA. The transformation efficiencies of B. flavum MJ233C and C. glutamicum ATCC 31831 increased nearly 10( 3)-fold when plasmid DNA was isolated from the recipient strain itself or from a damdcm Escherichia coli mutant, as compared with DNA passed through a modification-proficient E. coli strain. These results sugge st the presence of a methyl-specific restriction system in certain str ains of coryneform bacteria. In addition, electroporation conditions w ere optimized.