ACUTE STERILE INFLAMMATION - CORRELATION BETWEEN CELLULAR-CHANGES ANDEXTRAMEDULLARY-PRODUCED REGULATORS INVIVO

Rats with polyvinylpyrrolidone (PVP)-induced sterile inflammation were used as a model in vivo for investigation of granulopoiesis and extra medullary-produced regulators. The data obtained demonstrated the inva sion of massive numbers of granulocytes at the site of inflammation (p eritoneal cavity) during the first 24 h of the acute phase of inflamma tion. To meet the organism's needs for granulocytes the activation of granulopoiesis in bone marrow occurred simultaneously. Accelerated pro duction of granulocytic cells is manifested by involvement of granuloc ytic proliferative compartment in various stages of differentiation (C FU-GM and morphologically recognizable proliferative granulocytes - PG ). Together with cellular changes within the granulocytic cells line, the changes in the content of investigated regulators influencing gran ulopoiesis were observed. At different time intervals the levels of in terleukin-6 (IL-6), colony-stimulating activity (CSA), and granulocyti c stimulating activity (GSA) were increased locally at the site of inf lammation as well as in serum. The data obtained provide evidence that inducible granulopoiesis during the acute phase of inflammation is un der the control of extramedullary-produced regulators, thus confirming their role in the regulation of granulocytic production in vivo.