THE HUMAN TESTIS-DETERMINING FACTOR SRY BINDS A NUCLEAR FACTOR-CONTAINING PDZ PROTEIN-INTERACTION DOMAINS

Citation
F. Poulat et al., THE HUMAN TESTIS-DETERMINING FACTOR SRY BINDS A NUCLEAR FACTOR-CONTAINING PDZ PROTEIN-INTERACTION DOMAINS, The Journal of biological chemistry, 272(11), 1997, pp. 7167-7172
Citations number
26
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
272
Issue
11
Year of publication
1997
Pages
7167 - 7172
Database
ISI
SICI code
0021-9258(1997)272:11<7167:THTFSB>2.0.ZU;2-A
Abstract
The human Y-linked testis determining gene SRY encodes a protein with a DNA binding domain from the high mobility group box family, To date, no function has been assigned to amino acid sequences located outside this DNA binding moth, Here, we identify in a yeast two-hybrid screen a PDZ protein termed SIP-1, as an interacting protein with human SRY, In vitro, biochemical analysis, immunoprecipitation experiments, as w ell as expression of SIP-1 in human embryonic testis confirm that the two proteins can interact together, Interacting domains were mapped to the C-terminal seven amino acids of SRY and to the PDZ domains of SIP -1, respectively. We hypothesize that SIP-1 could connect SRY to other transcription factors providing SRY for its missing trans-regulation domain.