USING TOAD OOCYTES AS AN ULTRASENSITIVE SYSTEM FOR ESTIMATING THE BIOLOGICAL-ACTIVITY OF TRICHOSANTHIN

Authors
XIONG YZ ZHU G ZUO JK WANG YH
Citation
Yz. Xiong et al., USING TOAD OOCYTES AS AN ULTRASENSITIVE SYSTEM FOR ESTIMATING THE BIOLOGICAL-ACTIVITY OF TRICHOSANTHIN, Science in China. Series B, Chemistry, life sciences & earth sciences, 36(4), 1993, pp. 402-410
Citations number
16
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Science in China. Series B, Chemistry, life sciences & earth sciences → ACNP
ISSN journal
1001652X
Volume
36
Issue
4
Year of publication
1993
Pages
402 - 410
Database
ISI
SICI code
1001-652X(1993)36:4<402:UTOAAU>2.0.ZU;2-R
Abstract
A sensitive bioassay for estimating the activity of Trichosanthin was developed by using intraoocyte microinjection as the technique and ger minal vescicle breakdown as a marker. The GVBD inhibitory activity ass ay was million-fold more sensitive than the routinely used abortifacie nt activity assay. The detectable amount of Trichosanthin ranged from 0.5 to 100 pg. Since the least detectable dose was 0.5 pg, it made the assay suitable to analyse the microquantity of pure Trichosanthin use d for studying the structure and function. of the drug.