DETECTION OF ENTEROVIRAL RNA SEQUENCES IN DIFFERENT WATER SAMPLES

Authors
TOUGIANIDOU D BOTZENHART K
Citation
D. Tougianidou et K. Botzenhart, DETECTION OF ENTEROVIRAL RNA SEQUENCES IN DIFFERENT WATER SAMPLES, Water science and technology, 27(3-4), 1993, pp. 219-222
Citations number
5
Categorie Soggetti
Water Resources","Environmental Sciences","Engineering, Civil
Journal title
Water science and technology → ACNP
ISSN journal
02731223
Volume
27
Issue
3-4
Year of publication
1993
Pages
219 - 222
Database
ISI
SICI code
0273-1223(1993)27:3-4<219:DOERSI>2.0.ZU;2-N
Abstract
Viruses were isolated from different water samples by Sterivex-Filtrat ion. The nucleic acids were isolated in the filter unit and purified b y phenol-chloroform extraction and ethanol precipitation. Reverse tran scription and polymerase chain reaction (RT/PCR) were performed with p rimer pairs complementary to sequences of the enteroviral 5' noncoding region. Amplified sequences were detected by hybridisation with an ol igonucleotide complementary to a part of the PCR product. The test sys tem seems to be sensitive and specific in the detection of enteroviral RNA.