INACTIVATION OF HEPATITIS-A VIRUS AND MS2 BY OZONE AND OZONE-HYDROGENPEROXIDE IN BUFFERED WATER

The comparative inactivation of highly purified hepatitis A virus (HAV ) and MS2 by 1 mg H2O2/L, 2.0 and 0.4 mg O3/L, and 2.0 mg O3/L plus 0. 6, 1.0, or 1.6 mg H2O2/L, at 3-10-degrees-C, in 0-01 M phosphate buffe r (pH 6-10) was determined. Both HAV and MS2 were completely inactivat ed (3.9-6 log10) within 5 seconds in ozone solutions. Hydrogen peroxid e did not inactivate MS2, but di inactivate 95% of the HAV. Both HAV a nd MS2 were completely (3.3-5.5 log) inactivated within 5 seconds in t he oxidant mixtures at pH 6-8, but MS2 survival exceeded that of HAV a t pH 10. MS2 was found to be a good model for predicting HAV inactivat ion by ozone and mixtures of ozone and hydrogen peroxide when both vir uses are purified in the same manner.