Ts. Lawruk et al., QUANTIFICATION OF CYANAZINE IN WATER AND SOIL BY A MAGNETIC PARTICLE-BASED ELISA, Journal of agricultural and food chemistry, 41(5), 1993, pp. 747-752
A competitive enzyme-linked immunosorbent assay (ELISA) for the quanti
tation of cyanazine in water and soil was developed using a novel magn
etic particle solid phase. Cyanazine was covalently attached to a bovi
ne serum albumin carrier, and the resulting herbicide-protein conjugat
e was used in rabbits to produce polyclonal antibodies specific for cy
anazine. Specificity studies indicate that the antibody can distinguis
h cyanazine from other structurally similar triazine herbicides, inclu
ding atrazine. This ELISA has a limit of detection of 0.035 parts per
billion (ppb, ng/mL) in water and 3.5 ppb in soil. The standard curve
allows quantitation up to 3.0 ppb in water and 300 ppb in soil. The EL
ISA compares favorably with GC measurements in the analysis of water s
amples (r = 0.964). Recoveries from fortified soils averaged 86% using
a 24-h extraction with methanol/water (75:25 v/v).