ANALYSIS OF FUMONISIN B1 IN RODENT FEED BY GRADIENT ELUTION HPLC USING PRECOLUMN DERIVATIZATION WITH FMOC AND FLUORESCENCE DETECTION

A new, sensitive, arid stable method has been developed for the analys is of fumonisin B1 in rodent feed. Fumonisin B1, which is the major fu monisin metabolite produced by the fungus Fusarium moniliforme, has be en implicated in human and animal diseases. Fumonisin B1 was extracted from spiked rodent feed with acetonitrile/water (50/50) and then clea ned up with tandem C18 Sep-Pak Vac and strong anion-exchange (SAX) col umns. Fumonisin B1 was eluted off the SAX column with 1% acetic acid i n methanol and quantitated via gradient elution HPLC using precolumn d erivatization with FMOC and fluorescence detection. The minimum detect able amount in the rodent feed was 0.2 ppm. Recovery values in spiked rodent feed averaged 83% over the range 2-20 ppm.