HEMOLYSIS IN ADULT AND NEONATAL ERYTHROCYTES CAUSED BY AUTOXIDATION OF LIPID EMULSION (INTRALIPID)

Citation
S. Kljuchnikov et al., HEMOLYSIS IN ADULT AND NEONATAL ERYTHROCYTES CAUSED BY AUTOXIDATION OF LIPID EMULSION (INTRALIPID), Acta paediatrica, 82(4), 1993, pp. 348-351
Citations number
31
Categorie Soggetti
Pediatrics
Journal title
ISSN journal
08035253
Volume
82
Issue
4
Year of publication
1993
Pages
348 - 351
Database
ISI
SICI code
0803-5253(1993)82:4<348:HIAANE>2.0.ZU;2-I
Abstract
Lipid emulsion (Intralipid) causes free radical-mediated damage to hum an cells in vitro. Incubation with 0.44% Intralipid for 17 h caused 40 .3 +/- 3.8% haemolysis in adult human erythrocytes and 26.5 +/- 8.1% i n erythrocytes from term newborns (p = 0.0001). In adult erythrocytes mean corpuscular volume increased 68.7 +/- 8.2% and in newborn erythro cytes 54.8 +/- 10.4% (p = 0.0012). Initial concentrations of reduced g lutathione in adult and newborn erythrocytes were 65.1 +/- 2.5 and 62. 1 +/- 4.0 mg/dl, respectively (ns); after incubation, glutathione conc entrations were 21.0 +/- 4.0 and in 25.7 +/- 5.2 mg/dl in adult and ne wborn erythrocytes, respectively (p = 0.0004). After incubation the co ncentrations of thiobarbituric acid reactive material and conjugated d ienes in newborn erythrocytes (2.8 +/- 0.2 muM and 0.223 +/- 0.019 OD 233, respectively) were higher than those of adult erythrocytes (2.1 /- 0.4 muM and 0.138 +/- 0.012 OD 233) (p = 0.0001). In both adult and newborn erythrocytes, the effects of Intralipid were significantly in hibited by 0.6 mM desferoxamine or 8 mM sodium etidronate. Despite hig her susceptibility to lipid peroxidation of the cell membrane, newborn erythrocytes are more resistant than adult erythrocytes to free radic al-mediated effects such as depletion of intracellular glutathione, ce ll swelling and haemolysis.