THE VALUE OF PERITONEAL-FLUID HYPOXANTHINE BESIDES LACTIC-ACID IN DETECTION OF THE VASCULAR COMPROMISE OF INTESTINE

Hypoxanthine has been shown to be a sensitive and more specific measur e of hypoxia than lactate in several body fluids other than peritoneal fluid. An experimental study was conducted to determine the value of peritoneal fluid hypoxanthine besides lactic acid levels in detecting vascular compromise of the intestine. Thirty male guinea pigs were div ided in 3 groups each containing 10 animals. Groups comprised of sham operation control, simple intestinal obstruction and intestinal obstru ction with vascular compromise. Lactic acid and hypoxanthine levels we re determined in peritoneal lavage effluents. Peritoneal fluid lactic acid levels increased after 4 hours of intestinal obstruction with vas cular compromise (p < 0.01) but not in simple intestinal obstruction a nd sham operation control groups. No detectable amount of hypoxanthine could be found in any of the fluid samples. It is concluded that unli ke in other body fluids hypoxanthine is not present in peritoneal flui d and does not become evident after vascular compromise of the intesti ne.