EFFECTS OF KINDLING ON SUBSEQUENT LEARNING, MEMORY, BEHAVIOR, AND SEIZURE SUSCEPTIBILITY

To determine the long-term effects of seizures on the developing brain we kindled 20-, 40-, and 60-day-old rats to stage 5 seizures and then elicited an additional 15 seizures using the same kindling stimulatio n. At age 80 days, all animals that reached stage 5 kindling, and thei r respective age-matched controls, underwent behavioral testing using the Morris water maze, open field test, and handling test. Prior to eu thanasia the animals had seizure threshold tested using flurothyl inha lation. No differences were noted in time to platform in the water maz e or activity level in the open field test between the kindled rats an d controls in any of the three age groups. Rats kindled at age 20 and 40 were more emotional than the controls in the handling test. In the flurothyl inhalation test, rats kindled at 40 and 60 days of age had a shorter latency to all seizure stages than the controls. These result s demonstrate that while kindling results in no alteration of learning , memory, or activity level, it does result in altered emotionality an d activity level in immature animals, as well as reduced seizure thres hold in pubescent and mature rats. The animal model used appears to be an important variable in determining the long-term effects of seizure s.