PURIFICATION AND ANTICANCER ACTIVITY OF CYTOTOXIN-14 FROM VENOM OF NAJA-NAJA-ATRA

Authors
ZHONG XY LIU GF WANG QC
Citation
Xy. Zhong et al., PURIFICATION AND ANTICANCER ACTIVITY OF CYTOTOXIN-14 FROM VENOM OF NAJA-NAJA-ATRA, Zhongguo yaoli xuebao, 14(3), 1993, pp. 279-282
Citations number
11
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
Zhongguo yaoli xuebao → ACNP
ISSN journal
02539756
Volume
14
Issue
3
Year of publication
1993
Pages
279 - 282
Database
ISI
SICI code
0253-9756(1993)14:3<279:PAAAOC>2.0.ZU;2-6
Abstract
Ion-chromatography of lyophilized cobra (Naja naja atra) venom on SP-S ephadex C-50 yielded 14 fractions, of which 7, 8, 9, and 10 possessed neurotoxic actions and 11, 12, 13, and 14 had cytotoxic activities. Ch romatography of fraction 14 on SP-Sephadex C-25 gave cytotoxin-14. It was homogeneous on polyacrylamide gel electrophoresis with m = 7448 Da . It was a typical cytotoxin without phospholipase A2 activity and had a selective cytotoxic action on human cancer cell lines.