PROTECTIVE EFFECT ON ISCHEMIC DEPLETION OF NUCLEOTIDE PHOSPHATES IN HEART, BRAIN, AND KIDNEY BY CYCLOPHOSPHAMIDE

The levels of ATP, ADP, and AMP in heart, brain, and kidney suffering from 10-min ischemia after decapitation in rats were determined by a m odified reverse-phase HPLC set with uv detection. The ischemic depleti on of ATP was alleviated and the total amount of high energy phosphate s was markedly reduced by the treatment of po cyclophosphamide 20 and 100 mg . kg-1 X 3 d. The protective effect on depleting the total amou t of high energy phosphates which was better preserved than ATP in isc hemic organs by cyclophosphamide was evidenced in a dose-related manne r. Cyclophosphamide induced leukopenia in circulating blood. Two reaso ns for the anti-arrhythmic effect of cyclophosphamide are suggested: 1 ) the depletion of leukocyte reduced the plugging effect of neutrophil in myocardial capillaries; 2) blocking the K(ATP) channel by elevatin g ATP level in myocardium.