A NOVEL VERY SMALL SUBUNIT OF A SELENIUM-CONTAINING [NIFE] HYDROGENASE OF METHANOCOCCUS-VOLTAE IS POSTTRANSLATIONALLY PROCESSED BY CLEAVAGEAT A DEFINED POSITION

A coenzyme-F420 non-reducing [NiFe] hydrogenase was isolated from Meth anococcus voltae. It consists of three subunits. They are the products of the previously identified genes vhuA, vhuG and vhuU. The vhuU gene product is of only 25 amino acids. This novel very small hydrogenase subunit contains selenocysteine within a conserved amino-acid sequence previously shown to be involved in Ni coordination. The subunit is sh orter than the predicted primary gene product and is therefore apparen tly post-translationally processed.