RELATIONSHIPS BETWEEN THE THYROID AND SOMATOTROPIC AXES IN STEERS .2.EFFECTS OF THYROID STATUS ON PLASMA-CONCENTRATIONS OF INSULIN-LIKE GROWTH FACTOR-I (IGF-I) AND THE IGF-I RESPONSE TO GROWTH-HORMONE

Three studies assessed the effect of thyroid status on regulation of p lasma IGF-I in cattle. First, four Angus-Hereford steers (av wt 345 kg ) were fed 4 mg/d propylthiouracil daily for 35 d. With continued feed ing of PTU steers were sequentially injected with thyroxine (T4, 5 mg/ d, IM for 5 d) followed by triiodothyronine (T3, 2 mg/d, IM for 5 d). An injection of bovine pituitary growth hormone (GH, 0.1 mg/kg, IM) wa s given to each steer on day 35 of PTU, day 5 of T4 and again on day 5 of T3. PTU alone increased plasma thyroid stimulating hormone (TSH), decreased plasma T4 and T3 but had no influence on IGF-I. T3, but not T4, lowered plasma TSH, IGF-I and the IGF-I response to GH (P<.05). Ne xt, twelve bull calves (av wht 167 kg) were divided equally into two g roups. A control group was injected daily for five d with buffered sal ine; the experimental group was concurrently treated with T3 (5 mg/d, sc) for five d. Beginning the sixth day, all calves were injected with GH (0.1 mg/kg, IM daily) for three d with the respective buffer or T3 treatments continuing. Plasma IGF-I was depressed 29% by T3. The incr emental area under the three-d response curve was less (P<.03) in T3 c attle. A growth trial was conducted in which twenty-four Angus x Heref ord steers were injected daily with T3(2 mg/kg, bi-daily x 56 d) or im planted with Synovex-S (S) in a 2 x 2 factorial arrangement. Synovex i ncreased empty body protein gain (EBPG) and plasma IGF-I 15.5 and 27.9 % (P<.01), respectively; T3 decreased EBPG and plasma IGF-I 13.9 and 1 5.1% (P<.07), respectively, in steers which maintained suppression in plasma TSH. The data support the conclusion that elevated T3 decreases plasma IGF-I, in part, through a diminished GH-responsiveness and ana bolic treatments such as S can reverse the effects of excess T3.