THERMODYNAMIC STABILITY AND DRUG-BINDING PROPERTIES OF OLIGODEOXYRIBONUCLEOTIDE DUPLEXES CONTAINING 3-DEAZAADENINE - THYMINE BASE-PAIRS

Authors
LEVER C LI X COSSTICK R EBEL S BROWN T
Citation
C. Lever et al., THERMODYNAMIC STABILITY AND DRUG-BINDING PROPERTIES OF OLIGODEOXYRIBONUCLEOTIDE DUPLEXES CONTAINING 3-DEAZAADENINE - THYMINE BASE-PAIRS, Nucleic acids research, 21(8), 1993, pp. 1743-1746
Citations number
20
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
21
Issue
8
Year of publication
1993
Pages
1743 - 1746
Database
ISI
SICI code
0305-1048(1993)21:8<1743:TSADPO>2.0.ZU;2-4
Abstract
We have used ultraviolet melting techniques to study the effect on sta bility of incorporating the nucleoside analogue 2'-deoxy-3-deazaadenos ine (d(3C)A) into the duplex 5'-d(CGCAATCG)-3'-d(GCGTTAGC). Our result s demonstrate that the successive replacement of dA by d(3C)A increasi ngly destabilises the duplex. The destabilising effect of this analogu e is considerably enhanced as the pH is lowered and the results are co nsistent with protonation of 3-deazaadenine (probably at N-1) contribu ting to duplex destabilisation. Surprisingly, the incorporation of d(3 C)A does not significantly affect the binding of distamycin-A.