CRYOPRESERVATION OF OIL PALM (ELAEIS-GUINEENSIS JACQ) SOMATIC EMBRYOSINVOLVING A DESICCATION STEP

Citation
D. Dumet et al., CRYOPRESERVATION OF OIL PALM (ELAEIS-GUINEENSIS JACQ) SOMATIC EMBRYOSINVOLVING A DESICCATION STEP, Plant cell reports, 12(6), 1993, pp. 352-355
Citations number
18
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
07217714
Volume
12
Issue
6
Year of publication
1993
Pages
352 - 355
Database
ISI
SICI code
0721-7714(1993)12:6<352:COOP(J>2.0.ZU;2-L
Abstract
The standard cryopreservation process previously developed for oil pal m clones using shiny white, finger-like somatic embryos could be appli ed in some cases to standard cultures. Its efficiency was markedly imp roved by completing the 7-day pregrowth period on 0.75 M sucrose by an additional dehydration period carried out either by placing the embry os in the air current of the laminar flow cabinet or in an air tight b ox containing silica gel. This improved process was successfully appli ed to 7 different clones. It will facilitate the routine use of cryopr eservation for oil palm cultures.