THE USE OF RANDOM AMPLIFIED POLYMORPHIC DNAS TO FINGERPRINT APPLE GENOTYPES

Authors
MULCAHY DL CRESTI M SANSAVINI S DOUGLAS GC LINSKENS HF MULCAHY GB VIGNANI R PANCALDI M
Citation
Dl. Mulcahy et al., THE USE OF RANDOM AMPLIFIED POLYMORPHIC DNAS TO FINGERPRINT APPLE GENOTYPES, Scientia horticulturae, 54(2), 1993, pp. 89-96
Citations number
7
Categorie Soggetti
Horticulture
Journal title
Scientia horticulturae → ACNP
ISSN journal
03044238
Volume
54
Issue
2
Year of publication
1993
Pages
89 - 96
Database
ISI
SICI code
0304-4238(1993)54:2<89:TUORAP>2.0.ZU;2-J
Abstract
Twenty-five accessions of apple, representing eight cultivars ('Golden Delicious', 'Delicious', 'Gala', 'Jonagold', 'Jonathan', 'Florina', ' Fior di Cassia', and 'Imperatore Dallago') have been characterized wit h Random Amplified Polymorphic DNAs (RAPD). The reliability of the met hod was tested by analyzing separate scions of the same clone and also by comparing different accessions of the same cultivar. Using two sep arate ten bp primers, it was possible to obtain a distinctive fingerpr int for each of the cultivars. The method is simple, rapid and should provide a useful system for documenting the identity of clonal materia l.