Pl. Daluz et al., ANTAGONIZING THE HYDROXYL ION FREE-RADICAL (HO.) DOES NOT ABOLISH REPERFUSION VENTRICULAR-FIBRILLATION IN ANESTHETIZED DOGS, Brazilian journal of medical and biological research, 26(5), 1993, pp. 477-489
1. The hypothesis that the hydroxyl ion free radical, HO., derived fro
m O2 plays a pivotal role in the development of reperfusion ventricula
r fibrillation was tested in 63 anesthetized mongrel dogs of either se
x weighing 14 +/- 7 kg submitted to 90-min coronary occlusion followed
by 60-min reperfusion. 2. OH. was blocked by the iron chelator defero
xamine (DF,500 mg) and by dimethylthiourea (DMTU, 500 mg/kg), a HO. sc
avenger both given iv over 30 min before reperfusion. 3. The frequency
of reperfusion ventricular fibrillation was similar in all animals, i
.e., 7/27 (26%) control dogs, 7/23 (30%) DF-treated dogs and 3/13 (23
%) DMTU-treated dogs. Arterial pressure, heart rate and double product
were not significantly different among the three groups during occlus
ion or reperfusion. The hemodynamic variables were also similar among
dogs that fibrillated and those that did not. Likewise, extent of isch
emic areas and necrosis was similar among the three experimental group
s, with the control values being 34 +/- 4% and 14 +/- 5%, respectively
. 4. We conclude that OH. does not play a major role in the induction
of reperfusion ventricular fibrillation in the anesthetized dog with i
schemia/necrosis.