IMMOBILIZATION OF PENICILLIN ACYLASE ON COPOLYMER OF BUTYL ACRYLATE AND ETHYLENE-GLYCOL DIMETHACRYLATE

The effects of glutaraldehyde, enzyme concentrations and reactants vol umes, ionic strength, pH value and carrier particle diameter on immobi lization of penicillin acylase onto acrylic carriers were studied. The activity of immobilized enzyme preparations was also studied over a r ange of pH values and temperatures and thermal and pH stabilities were determined. The use of the immobilized preparation for penicillin G h ydrolysis in a batch reactor was investigated. The immobilized enzyme gave a significant reduction in hydrolysis time compared to hydrolysis by the native enzyme.