Aa. Abouzeid et Sm. Khoja, UTILIZATION OF DATES IN THE FERMENTATIVE FORMATION OF CITRIC-ACID BY YARROWIA-LIPOLYTICA, Zentralblatt fur Mikrobiologie, 148(3), 1993, pp. 213-221
Yarrowia lipolytica NRRL Y-1095 was found to be the most potent organi
sm for citric acid formation. Utilization of different Barni date-coat
sugar extract concentrations in the fermentation medium as carbon sou
rce was found to be good carbon in concentration of 25 mg/ml. Date-see
d hydrolysate as nitrogen source for citric acid formation was suitabl
e due to the presence of miscellaneous nitrogen sources, especially am
ino acids. The yield of citric acid was increased with the increase of
phosphorus concentration reaching its maximum when the medium was sup
plemented with about 1.0 mg KH2PO4/ml. On using different oxaloacetic
acid concentrations in the fermentation medium, it was found that citr
ic acid was increased with the increase of oxaloacetic acid concentrat
ion reaching its maximum when the medium was supplemented with 0.4 mg/
ml.