APPLICATION OF DNA FILTER HYBRIDIZATION AND PCR TO DISTINGUISH BETWEEN HUMAN AND NONHUMAN TISSUES OF POOR QUALITY

Citation
R. Mullenbach et al., APPLICATION OF DNA FILTER HYBRIDIZATION AND PCR TO DISTINGUISH BETWEEN HUMAN AND NONHUMAN TISSUES OF POOR QUALITY, International journal of legal medicine, 105(6), 1993, pp. 307-309
Citations number
10
Categorie Soggetti
Pathology
ISSN journal
09379827
Volume
105
Issue
6
Year of publication
1993
Pages
307 - 309
Database
ISI
SICI code
0937-9827(1993)105:6<307:AODFHA>2.0.ZU;2-V
Abstract
The present report describes a novel approach for the identification o f human or non-human specimens after long-term storage in a badly pres erved state. The application of the PCR-technique (polymerase chain re action) using human-specific primers as well as Southern blot (filter) hybridization of the sample DNA to a primate-specific DNA probe enabl ed us to extend the positive identification beyond the limits of conve ntional methods such as serological or morphological examinations.