JUVENILE-HORMONE BIOSYNTHESIS AND DIAPAUSE TERMINATION IN BOMBUS-TERRESTRIS

Citation
M. Larrere et al., JUVENILE-HORMONE BIOSYNTHESIS AND DIAPAUSE TERMINATION IN BOMBUS-TERRESTRIS, INVERTEBRATE REPRODUCTION & DEVELOPMENT, 23(1), 1993, pp. 7-14
Citations number
19
Categorie Soggetti
Reproductive Biology",Zoology
ISSN journal
07924259
Volume
23
Issue
1
Year of publication
1993
Pages
7 - 14
Database
ISI
SICI code
0792-4259(1993)23:1<7:JBADTI>2.0.ZU;2-Z
Abstract
Corpora allata from female Bombus terrestris, incubated in a medium co ntaining L-[methyl-H-3]methionine, released three radiolabelled produc ts, one of which was identified as JH-III by RPLC analysis. Two others did not correspond to any JH analogues or JH-related compounds, and t hey remain unidentified. Exogenous farnesol stimulated JH-III biosynth esis and suppressed the synthesis of the two unidentified products. Co rpora allata activity was monitored throughout and after adult diapaus e. Rates of JH-III biosynthesis were low during prediapause and diapau se. Corpora allata reactivation occurred several days after emergence from soil and thus seemed not to be involved in diapause termination p rocesses. Experimental diapause breakdown revealed two phases in imagi nal Bombus diapause: diapause development and post-diapause quiescence . Carbon dioxide narcosis associated with 20-degrees-C, 13L/11D condit ions provided rapid and reliable diapause termination in both diapause phases. The mode of CO2 narcosis action is not mediated by corpora al lata reactivation and remains to be elucidated.