EFFECTS OF CARBACHOL AND GASTRIN ON C-14 AMINOPYRINE ACCUMULATED IN RABBIT GASTRIC GLANDS AND CELLS

The present study examines the possible existence of a mechanism regul ating emptying of the secretory canaliculi content of the parietal cel l and the possible effects of carbachol and gastrin on such a mechanis m. In rabbit gastric glands stimulated with carbachol, C-14-aminopyrin e accumulation reached a maximum after 15 min and then started to decr ease. This decrease was not accompanied by a decrease in oxygen consum ption, nor was any decrease of accumulated C-14-aminopyrine seen in di spersed gastric cells. In glands but not in cells stimulated with hist amine together with 3-isobutyl-1-methylxanthine (IMX), carbachol induc ed a reduction in the accumulated C-14-aminopyrine content, whereas th e effect of gastrin was less pronounced, The carbachol-induced reducti on was counteracted by atropine but was not accompanied by a decrease in oxygen consumption. It is suggested that there exists a mechanism t hat controls the emptying of the secretory canaliculi content of the p arietal cell, and that carbachol, in addition to stimulating acid prod uction, also contributes to this emptying. Paracrine factors may be in volved in this latter mechanism.