SEX DETERMINATION OF MURINE AND BOVINE EMBRYOS USING CYTOTOXICITY ANDIMMUNOFLUORESCENCE ASSAYS

Authors
DELIMA VFMH MOREIRA CA DEBEM AR JORGE W
Citation
Vfmh. Delima et al., SEX DETERMINATION OF MURINE AND BOVINE EMBRYOS USING CYTOTOXICITY ANDIMMUNOFLUORESCENCE ASSAYS, Theriogenology, 39(6), 1993, pp. 1343-1352
Citations number
33
Categorie Soggetti
Veterinary Sciences
Journal title
Theriogenology → ACNP
ISSN journal
0093691X
Volume
39
Issue
6
Year of publication
1993
Pages
1343 - 1352
Database
ISI
SICI code
0093-691X(1993)39:6<1343:SDOMAB>2.0.ZU;2-X
Abstract
Murine and bovine embryos were sexed by using polyclonal and monoclona l anti-H-Y antibodies in cytotoxic and immunofluorescence assays. The specificity of 3 different sources of antibodies are compared. Sexing was accomplished by complement-mediated cytotoxicity or by H-Y antigen detection using a anti-mouse Ig fluorescein conjugate. Cytogenetic as certainment for correct sex identification yielded a score of about 85 % in both species, while the immunofluorescence assay yielded a score of 83%.