SIMULTANEOUS EXPRESSION BY PORCINE AORTA ENDOTHELIAL-CELLS OF GLYCOSPHINGOLIPIDS BEARING THE MAJOR EPITOPE FOR HUMAN XENOREACTIVE ANTIBODIES (GAL-ALPHA-1-3GAL), BLOOD-GROUP-H DETERMINANT AND N-GLYCOLYLNEURAMINIC ACID
D. Bouhours et al., SIMULTANEOUS EXPRESSION BY PORCINE AORTA ENDOTHELIAL-CELLS OF GLYCOSPHINGOLIPIDS BEARING THE MAJOR EPITOPE FOR HUMAN XENOREACTIVE ANTIBODIES (GAL-ALPHA-1-3GAL), BLOOD-GROUP-H DETERMINANT AND N-GLYCOLYLNEURAMINIC ACID, Glycoconjugate journal, 13(6), 1996, pp. 947-953
Glycosphingolipids were isolated from primary cultures of porcine endo
thelial cells labelled with C-14-galactose or C-14-glucosamine. They w
ere characterized by their mobility on thin layer chromatogram, their
sensitivity to exoglycosidases, and their labelling with antibodies. I
n addition to the major glycosphingolipids, globotetra- and globotriao
sylceramide, minor ones were identified as penta- and heptaglycosylcer
amide of the neolactoseries terminated by either Gal alpha 1-3Gal- (xe
noreactive epitope) or Fuc alpha 1-2Gal- (H determinant). Two ganglios
ides were found, GM3 and GD3, and N-glycolylneuraminic acid was their
major sialic acid. Therefore, porcine endothelial cells differ from hu
man endothelial cells by expression of glycosphingolipids that are abs
ent in man: two Gal alpha 1-3Gal-terminated glycolipids recognized by
human natural antibodies, and two N-glycolylneuraminic acid-terminated
gangliosides which are potent immunogens.