HIGH-THROUGHPUT DNA SEQUENCING USING AN AUTOMATED ELECTROPHORESIS ANALYSIS SYSTEM AND A NOVEL SEQUENCE ASSEMBLY PROGRAM

The combination of high-resolution scanning, image-databasing technolo gy and sequence assembly software makes it possible to assemble contig uous overlapping sequences of DNA in a fraction of the tune required b y manual methods. This paper describes and evaluates an assembly progr am that rapidly generates contigs from scanned images and provides a u nique ability to verify disagreements between overlapping strands or f ragments of complementary DNA sequences on-screen, further increasing sequencing throughput.