APPLICATION OF APPARENT CONTENT CURVES TO MOLECULAR FLUORESCENCE DETERMINATION OF AN ANALYTE IN THE PRESENCE OF ONE INTERFERENT

The resolution of binary mixtures that have spectral interferences in molecular fluorescence is carried out using apparent content curves. T he use of this mathematical method permits the identification of the n ature of the interferent in binary analyte-interferent mixtures. The r esolution of binary mixtures is however carried out without using a st andard interference solution. The method is applied to the resolution of the binary mixtures fluorescein-acridine and fluorescein-eosin. The results are satisfactory in both cases.