DETECTION OF LEGIONELLA SPP IN COOLING-TOWER WATER BY THE POLYMERASE CHAIN-REACTION METHOD

Authors
KOIDE M SAITO A KUSANO N HIGA F
Citation
M. Koide et al., DETECTION OF LEGIONELLA SPP IN COOLING-TOWER WATER BY THE POLYMERASE CHAIN-REACTION METHOD, Applied and environmental microbiology, 59(6), 1993, pp. 1943-1946
Citations number
11
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
59
Issue
6
Year of publication
1993
Pages
1943 - 1946
Database
ISI
SICI code
0099-2240(1993)59:6<1943:DOLSIC>2.0.ZU;2-M
Abstract
The presence of Legionella spp. in cooling tower water was investigate d by using the polymerase chain reaction. Total Legionella spp. detect ion was performed with 20-mer 5S rRNA complementary DNA sequence prime rs, and specific Legionella pneumophila detection was performed with 2 0-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Of 27 cooling tower water samples, 25 were positive for Legi onella spp., and 14 of these contained L. pneumophila.