Rhodococcus strains were screened for their ability to degrade the her
bicide atrazine. Only rhodococci that degrade the herbicide EPTC (s-et
hyl-dipropylthiocarbamate) metabolized atrazine. Rhodococcus strain TE
1 metabolized atrazine under aerobic conditions to produce deethyl- an
d deisopropylatrazine, which were not degraded further and which accum
ulated in the incubation medium. The bacterium also metabolized the ot
her s-triazine herbicides propazine, simazine, and cyanazine. The N de
alkylation of triazine herbicides by Rhodococcus strain TE1 was associ
ated with a 77-kb plasmid previously shown to be required for EPTC deg
radation.