TRANSDUCTION OF A PLASMID CARRYING THE COHESIVE END REGION FROM LACTOCOCCUS-LACTIS BACTERIOPHAGE PHI-LC3

Authors
BIRKELAND NK HOLO H
Citation
Nk. Birkeland et H. Holo, TRANSDUCTION OF A PLASMID CARRYING THE COHESIVE END REGION FROM LACTOCOCCUS-LACTIS BACTERIOPHAGE PHI-LC3, Applied and environmental microbiology, 59(6), 1993, pp. 1966-1968
Citations number
31
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
59
Issue
6
Year of publication
1993
Pages
1966 - 1968
Database
ISI
SICI code
0099-2240(1993)59:6<1966:TOAPCT>2.0.ZU;2-9
Abstract
Plasmids carrying the cohesive end region from temperate lactococcal b acteriophage phiLC3 could be packaged in vivo by phiLC3 and transduced into its host strain, Lactococcus lactis subsp. cremoris NCDO 1201. T he transduction frequencies were between 10(-4) and 10(-3) transducing particles per PFU, depending on the size of the phage DNA insert. Thi s transduction system is limited to only certain lactococcal strains. The phiLC3 cohesive site region (cos) appears to play an important rol e in plasmid transduction.