CHIRAL RESOLUTION OF DIOLS BY CAPILLARY ELECTROPHORESIS USING BORATE-CYCLODEXTRIN COMPLEXATION

In this study, the chiral separation of compounds with a cis-diol stru cture using an electrolyte containing berate and cyclodextrin (CD) is described. A dual mechanism involving both inclusion of the aromatic m oiety into the cavity of the CD and the formation of mixed berate comp lexes is assumed to be responsible for chiral recognition. It has been shown that the separation is greatly influenced by the structure of t he CD, the pH and the concentration of both the CD and berate. The met hod was optimized with some simple diol compounds and applied to quina zoline-(3H,1H)-4-on-2-thiones and tetrazol-5-thiones of potential phar maceutical interest.