USE OF CAPILLARY ELECTROPHORESIS AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR MONITORING OF GLYCOSYLATION OF THE PEPTIDES DALARGIN AND DESMOPRESSIN

Capillary electrophoresis (CE) and HPLC procedures have been developed and tested for monitoring of glycosylation of the peptide hormones da largin and desmopressin, permitting identification and determination o f the reaction products and establishing the reaction rate. Good separ ations have been obtained that can also be used for preparative purpos es, Reversed-phase HPLC employs a gradient of methanol in a mobile pha se of 0.1% trifluoroacetic acid, pH 2.2, with a diode-array UV detecti on. The CE systems involve 150 mM phosphoric acid, pH 1.9, or a micell ar system containing 50 mM dodecyl sulfate and 20 mM sodium tetraborat e, pH 9.2, both with UV photometric detection at 190 nm; the latter sy stem is less suitable for glycoconjugate isolation. Whereas CE is supe rior to HPLC in analytical application, HPLC is preferable for prepara tive purposes.