QUANTITATIVE-ANALYSIS OF FLAVONOIDS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Large variations were found in the effectiveness of five reversed-phas e high-performance liquid chromatography columns used for the analysis of free and conjugated flavonoids. The best results were obtained wit h C-18 Nova-Pak, C-18 Symmetry and C-18 Genesis columns but substantia l band broadening and peak asymmetry were observed when free flavonoid s were chromatographed on ODS-Hypersil and LiChrospher RP-18 supports. The Symmetry and Genesis columns provided gradient elution separation s of rutin, quercetin-3-glucoside, quercitrin, myricetin, luteolin, qu ercetin, apigenin, kaempferol and isorhamnetin. This procedure was use d for the quantitative analysis of endogenous flavones and flavonols i n acid hydrolysed extracts from onions and celery.