ONLINE SFE-GC FOR DETERMINATION OF PCBS IN HUMAN-MILK AND BLOOD-SERUM

On-line coupled supercritical fluid extraction and gas chromatography (SFE-GC) has been utilized for the determination of PCBs and other org anochlorine compounds in human milk and blood serum. The procedure inv olved preconcentration of the sample on C18-silica sorbent in an extra ction cell: after precipitation of the proteins up to 20 ml of human m ilk was concentrated on 0.5 g of sorbent. Serum (up to 5 ml) was appli ed to the C18 material without pretreatment. Basic alumina was utilize d as a selective adsorbent for lipids in the on-line SFE-GC system. Th e method was used to analyze milk and serum spiked with 0.5 and 10 ng of Aroclor 1260 and the results compared with those obtained by liquid - liquid extraction of serum.