SIMILARITY IN NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING NONTOXIC COMPONENT OF BOTULINUM TOXIN PRODUCED BY TOXIGENIC CLOSTRIDIUM-BUTYRICUM STRAIN BL6340 AND CLOSTRIDIUM-BOTULINUM TYPE-E STRAIN MASHIKE

Authors
FUJII N KIMURA K YOKOSAWA N OGUMA K YASHIKI T TAKESHI K OHYAMA T ISOGAI E ISOGAI H
Citation
N. Fujii et al., SIMILARITY IN NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING NONTOXIC COMPONENT OF BOTULINUM TOXIN PRODUCED BY TOXIGENIC CLOSTRIDIUM-BUTYRICUM STRAIN BL6340 AND CLOSTRIDIUM-BOTULINUM TYPE-E STRAIN MASHIKE, Microbiology and immunology, 37(5), 1993, pp. 395-398
Citations number
10
Categorie Soggetti
Microbiology,Immunology
Journal title
Microbiology and immunology → ACNP
ISSN journal
03855600
Volume
37
Issue
5
Year of publication
1993
Pages
395 - 398
Database
ISI
SICI code
0385-5600(1993)37:5<395:SINOTG>2.0.ZU;2-Q
Abstract
The complete nucleotide and deduced amino acid sequence of the nontoxi c component of botulinum type E progenitor toxin is determined in reco mbinant plasmid pU9BUH containing about 6.0 kb HindIII fragment obtain ed from chromosomal DNA of Clostridium butyricum strain BL6340. The op en reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weig ht calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are diff erent in the nontoxic component gene between C. butyricum strain BL634 0 and C. botulinum type E strain Mashike. This corresponds to the diff erence of 17 amino acid residues in these nontoxic component.