P-MALEIMIDOPHENYL ISOCYANATE - A NOVEL HETEROBIFUNCTIONAL LINKER FOR HYDROXYL TO THIOL COUPLING

Authors
ANNUNZIATO ME PATEL US RANADE M PALUMBO PS
Citation
Me. Annunziato et al., P-MALEIMIDOPHENYL ISOCYANATE - A NOVEL HETEROBIFUNCTIONAL LINKER FOR HYDROXYL TO THIOL COUPLING, Bioconjugate chemistry, 4(3), 1993, pp. 212-218
Citations number
36
Categorie Soggetti
Biology,Chemistry
Journal title
Bioconjugate chemistry → ACNP
ISSN journal
10431802
Volume
4
Issue
3
Year of publication
1993
Pages
212 - 218
Database
ISI
SICI code
1043-1802(1993)4:3<212:PI-ANH>2.0.ZU;2-2
Abstract
p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-l inking agent useful for thiol to hydroxyl coupling. Several maleimide- activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activatio n of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.