CORRELATION OF CONTRACTILE FUNCTION OF THE RABBIT CORPUS CAVERNOSUM WITH NADH FLUORESCENCE

Citation
Ja. Hypolite et al., CORRELATION OF CONTRACTILE FUNCTION OF THE RABBIT CORPUS CAVERNOSUM WITH NADH FLUORESCENCE, Pharmacology, 46(6), 1993, pp. 353-361
Citations number
27
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
ISSN journal
00317012
Volume
46
Issue
6
Year of publication
1993
Pages
353 - 361
Database
ISI
SICI code
0031-7012(1993)46:6<353:COCFOT>2.0.ZU;2-N
Abstract
The NADH/NAD ratio is a measure of potential metabolic energy in smoot h muscle tissue. Previous studies on bladder smooth muscle demonstrate d that during active contraction when energy utilization is high, NADH is rapidly oxidized to NAD resulting in a decrease in the ratio of NA DH/NAD. Intracellular systems utilizing ATP as an energy source are ch aracterized by changes in the NADH/NAD ratio. This ratio can be monito red simultaneously with changes in smooth muscle tissue tone using an optical fiber probe which continually monitors NADH fluorescence and c ontractile activity of the same smooth muscle preparation. The present study correlates alterations in the ratio of NADH/NAD with both spont aneous and induced contractions and relaxations in the rabbit corpora cavernosa. The results show a high degree of correlation between a dec rease in spontaneous fluorescence (decrease in the NADH/NAD ratio) and spontaneous contraction. An increase in tension was followed in time by a decrease in the NADH/NAD ratio. This was consistent for all strip s showing significant spontaneous activity. ATP caused a rapid decreas e in tension which was correlated with a decrease in fluorescence. The relative decrease in NADH fluorescence was proportional to the relati ve decrease in tension. Under basal conditions (0.8 g passive tension) ATP and nitroprusside stimulated a marked reduction in tension, but o nly ATP stimulated a substantial decrease in NADH fluorescence. Bethan echol and isoproterenol relaxed the corporal tissue to a relatively sm all degree which correlated with relatively small decreases in fluores cence. Methoxamine stimulated a substantial contraction of corporal sm ooth muscle which correlated with a rapid and significant decrease in NADH fluorescence. Following precontraction by methoxamine, ATP stimul ated a marked decrease in both tension and fluorescence. Nitroprusside and bethanechol similarly stimulated marked decreases in tension with only minor changes in NADH fluorescence. Isoproterenol induced a mino r decrease in tension with no significant change in NADH fluorescence from that of the basal response. The dissociation of the endothelium f rom the corporal tissue inhibited the response to bethanechol by 90% f or both fluorescence and tension while the response to ATP was virtual ly unaffected. In summary, the data indicate a strong correlation betw een stimulation of contraction by methoxamine and a decrease in the NA DH/NAD ratio; and strong relaxation of corporal smooth muscle by ATP a nd a decrease in the NADH/NAD ratio. Since these drugs have opposite e ffects on tension, this result may indicate that both contraction- and ATP- induced relaxation in the rabbit corpus cavernosa are active pro cesses and depend on the rapid utilization of cellular metabolic energ y. It appears that corporal relaxation mediated by nitroprusside is hi ghly efficient, utilizing significantly less intracellular energy and producing maximal relaxations.