DETERMINATION OF ASCORBIC-ACID BY ISOTACHOPHORESIS WITH REGARD TO ITSPOTENTIAL IN NEUROBLASTOMA THERAPY

Analytical capillary isotachophoresis was used to determine ascorbic a cid (AA) in different matrices (cell-free system, neuroblastoma cell e xtracts and urine). The system for purging bone marrow of neuroblastom a cells, including 6-hydroxydopamine (6-OHDA) and AA, was analysed wit h regard to the interaction of AA with 6-OHDA and its autoxidation pro duct, hydrogen peroxide. Furthermore, analyses concerning the uptake o f AA into neuroblastoma cells as well as its excretion in urine after uptake of large amounts were carried out.