PROSPECTS OF DISSOLVED ALBUMIN AS A CHIRAL SELECTOR IN CAPILLARY ZONEELECTROPHORESIS

It has been shown using as examples amino acids. mono- and dicarboxyli c acids that the enantioselectivity of dissolved albumin may be employ ed with advantage in capillary zone electrophoresis (CZE) separations. Slow changes in the enantioselectivity of albumin dissolved in weakly alkaline aqueous solutions can be avoided by a short mild heating of the solution. The efficiency of a separation system for a given solute in CZE is at least one order of magnitude higher than that in LC base d on bonded albumin. The good chiral separations obtained here and the high efficiency of CZE separation systems confirm that CZE separation s based on dissolved albumin as a chiral selector can compete with ana logous LC separations. Perfect qualitative agreement of both enantiose lective properties of albumin and factors affecting its enantioselecti vity in CZE and LC proves that the knowledge obtained in LC with bonde d albumin may be transferred to CZE separation systems.