HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY GEL-ELECTROPHORESIS AS APPLIED TO ANTISENSE DNA

Authors
COHEN AS VILENCHIK M DUDLEY JL GEMBORYS MW BOURQUE AJ
Citation
As. Cohen et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY GEL-ELECTROPHORESIS AS APPLIED TO ANTISENSE DNA, Journal of chromatography, 638(2), 1993, pp. 293-301
Citations number
17
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography → ACNP
Volume
638
Issue
2
Year of publication
1993
Pages
293 - 301
Database
ISI
SICI code
Abstract
Reversed-phase and ion-exchange HPLC are compared with both slab gel a nd capillary gel electrophoresis tor the separation of antisense phosp horothioate oligomers. The chromatographic separations were found to b e markedly inferior to the electrophoretic separations, especially for oligomers greater than 20 bases in length. The potential of gel high- performance capillary electrophoresis for the analysis of phosphorothi oate analogues is shown.