CAPILLARY ELECTROPHORESIS OF O-PHENYLENEDIAMINE DERIVATIVES (QUINOXALINES) OF DICARBONYL SUGARS

Dicarbonyl sugars were separated by capillary electrophoresis as o-phe nylenediamine derivatives (quinoxalines). Tetrabutylammonium bromide w as used as a modifier; D-arabino-2-hexosulose (D-glucosone), D-lyxo-2- hexosulose (D-galactosone), 7-deoxy-L-galacto-2-hexosulose and 5-hydro xy-2,3-dioxohexanal served as standards. The separation procedure was optimized with respect to pH, buffer and solute concentrations. In mos t instances optimum separations were obtained in 75 mM borate buffer ( pH 11.0); 0.04 mg/ml was found to be the optimum sample concentration. The detection limit was ca. 0.1 pmol. Analyses of a mixture of fermen tation products or Maillard reaction products show the applicability o f the method.