O. Faff et al., LARGE-SCALE PRODUCTION AND PURIFICATION OF HUMAN RETROVIRUS-LIKE PARTICLES RELATED TO THE MOUSE MAMMARY-TUMOR VIRUS, FEMS microbiology letters, 109(2-3), 1993, pp. 289-296
Human retrovirus-like particles related to mouse mammary tumor virus (
MMTV) are secreted in a steroid-dependent manner by the breast cancer
cell line T47D. We report the successful large scale production and pu
rification of these particles from culture supernatants of T47D cells
and describe the experimental conditions established for this purpose.
Thus, mg amounts of particles were produced by large scale culturing
of T47D cells in an autoharvesting roller bottle system and purified b
y differential centrifugation and continuous flow ultracentrifugation
on density gradients with a 50% recovery and a 350-fold enrichment.