CHROMATIN STRUCTURES AND TRANSCRIPTION OF RDNA IN YEAST SACCHAROMYCES-CEREVISIAE

The chromatin structure of yeast ribosomal DNA was analyzed in vivo by crosslinking intact cells with psoralen. We found that in exponential ly growing cultures the regions coding for the 35S rRNA precursor fall into two distinct classes. One class was highly accessible to psorale n and associated with nascent RNAs, characteristic for transcriptional ly active rRNA genes devoid of nucleosomes, whereas the other class sh owed a crosslinking pattern indistinguishable from that of bulk chroma tin and was interpreted to represent the inactive rRNA gene copies. By crosslinking the same strain growing in complex or minimal medium, we have shown that yeast cells can modulate the proportion of active (no n-nucleosomal) and inactive (nucleosomal) rRNA gene copies in response to variations in environmental conditions which suggests that yeast c an regulate rRNA synthesis by varying the number of active gene copies , in contrast to the vertebrate cells studied so far. Whereas intergen ic spacers flanking inactive rRNA gene copies are packaged in a regula r nucleosomal array, spacers flanking active genes show an unusual cro sslinking pattern suggesting a complex interaction of regulatory tacto rs and histones with DNA.