ALLERGEN-INDUCED HISTAMINE-SECRETION ASSOCIATED WITH LACTATE PRODUCTION IN MAST-CELLS DETECTED BY H-1-NMR

Allergen-induced histamine secretion in mast cells, obtained from rats infected with Nippostrongylus brasiliensis, was detected by a H-1 NMR semi-selective excitation technique. Lactate production was also dete cted in association with histamine secretion as observed in mast cells stimulated with other nonimmunological agents, such as compound 48/80 , polymyxin B, and melittin. The assignment of the lactate resonance w as confirmed using lactate oxidase. Thus, H-1 NMR spectroscopy is a us eful method to study the activation of mast cells in allergic reaction .