EVALUATION OF SOURCES OF PARTIAL RESISTANCE TO MILDEW IN BARLEY USINGENZYME-LINKED-IMMUNOSORBENT-ASSAY AND OTHER ASSESSMENT METHODS

Twenty-nine spring barley genotypes ranging from primitive landraces t o modern cultivars were characterised for partial resistance to powder y mildew on detached leaves and in the field. Detached seedling and ad ult plant leaves were produced under spore-free glasshouse conditions and were assessed for infection frequency (IF) and then total mildew b iomass using enzyme-linked immunosorbent assay (ELISA), and thereby bi omass per colony (BMC), following controlled infection. Field plants w ere assessed visually for infection and for total mildew biomass using ELISA. Several genotypes were identified as having below average IF a nd BMC on both seedling and adult detached leaves and field scores of leaf area infected with mildew. IF at the seedling stage was highly co rrelated with IF at adult plant stage and with mildew biomass in the f ield. Adult plant IF was highly correlated with visual field scores. B MC data were often negatively correlated with other assessments.